| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.011720 |
| Chromosome: | chromosome 12 |
| Location: | 1235997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801365 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACTACCCTGGTCCAGGGGGAGCATGGCAGGGGCGGTGACGGGCAGCT |
| Internal bar code: | TGTTGTCTAGGGGTACAATATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3211 |
| LEAP-Seq percent confirming: | 36.5591 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGCTGGACTGGGATTC |
| Suggested primer 2: | CATACGATGGTACTGGGGGC |