Insertion junction: CLIP2.011734_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011734
Chromosome:chromosome 17
Location:801907
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g701551 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAAGCATAGAGGCGAAGGGACACACACAGACACAACCACCGTCGACGTG
Internal bar code:GGACCCGGTTAAGCGGGGACTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3255
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CACCACTACTGCTGCTGTCA
Suggested primer 2:CCTTGGTCACCCAGCCTTAG