Insertion junction: CLIP2.011744_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.011744
Chromosome:chromosome 3
Location:7516383
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g205400 (1 of 1) PF01344//PF07707 - Kelch motif (Kelch_1) // BTB And C-terminal Kelch (BACK) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTGTTCCTTATCTCGTGTTATTCCTAGCACACGCACACGCACACGCC
Internal bar code:TCAGTGCCACAATCAATCTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:61
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CACATCACCCCACCCTAACC
Suggested primer 2:CCTGTACGTCGTTGAGCAGT