| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011750 |
| Chromosome: | chromosome 6 |
| Location: | 2375560 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268228 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTCTACAGATATCTAGCTAGCAGGCCTCACATTATTGACGAGTGTTGCG |
| Internal bar code: | GTTTCTGGGTATCGATCTACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2357 |
| LEAP-Seq percent confirming: | 71.6667 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAGCGTTCAGTGGCTAC |
| Suggested primer 2: | GCATCTGCATCTGCTTCAGC |