| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011758 |
| Chromosome: | chromosome 12 |
| Location: | 4677945 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522450 | SEC31 | (1 of 1) K14005 - protein transport protein SEC31 (SEC31); COP-II coat subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTCTGCATCATTGCACGCGCGCGCAGGCCATCATCCGCGCCAACCTG |
| Internal bar code: | TAGCTCTTCGGTCGGTCATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2733 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGCAAGGACACGAAGGC |
| Suggested primer 2: | TGAGTTGCGCATGGTGTTTG |