| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.011763 |
| Chromosome: | chromosome 7 |
| Location: | 4135844 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342552 | UBC37 | E2 ubiquitin conjugating enzyme; (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAACCCAATTCCGTGGGGCCGTACAAGGTGAGAGAGCGATCATAAGT |
| Internal bar code: | GAGTAGGATAGGCATTACGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1240 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGTTGGGGGTGCAAAAG |
| Suggested primer 2: | CCCGGTGACTGAAGCTTGAT |