Insertion junction: CLIP2.011782_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.011782
Chromosome:chromosome 12
Location:664969
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g493250 FAP358 (1 of 2) PTHR24353//PTHR24353:SF37 - CYCLIC NUCLEOTIDE-DEPENDENT PROTEIN KINASE // PROTEIN KINASE DC1-RELATED; Flagellar Associated Protein 358 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGCCCACGTCGCCCTCCATGATCACACGCGCGCCCGCTGCACACATAC
Internal bar code:GAGGTATCTCTGGTGGGTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1609
LEAP-Seq percent confirming:93.75
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CACCCCACAGGCATTTTGTG
Suggested primer 2:TGGGGTTTCAACTTGGCCAT