| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011810 |
| Chromosome: | chromosome 12 |
| Location: | 9054127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544662 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCCGTCAGGAGTCGGGACTGCCGTGGTCGGGACAGCGCAAAGCCGCG |
| Internal bar code: | ATTAACACTGGACGTGTGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4789 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCGATCGGCTCTGGAC |
| Suggested primer 2: | CACTATCATTGTGGCGCACG |