| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.011814 |
| Chromosome: | chromosome 2 |
| Location: | 7621946 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147850 | CLPY2,HSLU2,HUV2 | (1 of 2) K03667 - ATP-dependent HslUV protease ATP-binding subunit HslU (hslU); Peptidase subunit of mitochondrial HslUV protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGAGGACTGTGAGCCCGGTGTTGCGCCCGCCGCAGCCAAGCAAGGCT |
| Internal bar code: | CCGGACGGGATAATCGTAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCCTACGATCGACCACTT |
| Suggested primer 2: | CGTTGTACCTTCCAGTCCCC |