Insertion junction: CLIP2.011814_6

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.011814
Chromosome:chromosome 2
Location:7621953
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147850 CLPY2,HSLU2,HUV2 (1 of 2) K03667 - ATP-dependent HslUV protease ATP-binding subunit HslU (hslU); Peptidase subunit of mitochondrial HslUV protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCATGAGGTTGCGCCGCCCGAATGACCCATTGACCATTGCGCCCCTCG
Internal bar code:CTTAGTTTTATGAAGAGAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1441
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGTACCTTCCAGTCCCC
Suggested primer 2:TCCCCTACGATCGACCACTT