Insertion junction: CLIP2.011816_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.011816
Chromosome:chromosome 6
Location:5276183
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284400 (1 of 1) K06695 - 26S proteasome regulatory subunit, ATPase 3, interacting protein (PSMC3IP) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACGGAAAGTGAGGCAGACGGGCCCAGCGCCGCCCAGCTCCGAGGGTTGT
Internal bar code:GCGTGTTTAGATAGGACAGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:394
LEAP-Seq percent confirming:82.7586
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGTGCTCTCTGACACGT
Suggested primer 2:AGCCCAGATCGAGCGTATTG