Insertion junction: CLIP2.011822_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.011822
Chromosome:chromosome 10
Location:3360058
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g443600 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCAACGACCGCGTTCCCTACCCTACCAGGTGCCGAAGTCCCACAAGGA
Internal bar code:CTCCGCCAAGACAGAGTGGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2786
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTGCAAAGTGTGGGAA
Suggested primer 2:CAAGCACAAGCACTGGTGAC