| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.011834 |
| Chromosome: | chromosome 2 |
| Location: | 5400571 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g113751 | (1 of 6) PTHR22916//PTHR22916:SF9 - GLYCOSYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGTGCCTGCCGTACGGGGCCCTCAGTCTGGGCCCATCATACCACTG |
| Internal bar code: | TTCGCCAATGGTTCCCGTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1163 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGAGGTGAGTACGACC |
| Suggested primer 2: | AACTACTTCAAGCGACCCGG |