| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.011844 |
| Chromosome: | chromosome 9 |
| Location: | 2628789 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g801018 | (1 of 1) PTHR11390//PTHR11390:SF21 - PROKARYOTIC DNA TOPOISOMERASE // DNA TOPOISOMERASE 3-ALPHA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGAATGTGACTTTCCCCCCCTCCCGACAGGTGATGGACGTGTGTCGC |
| Internal bar code: | TTAAGTGACTATGGATTGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAATGGGTGGGGGTTAG |
| Suggested primer 2: | CTCCCCTTTTCCCGTTCCTC |