| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.011855 |
| Chromosome: | chromosome 3 |
| Location: | 6731625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196150 | uL5m,MRPL5 | (1 of 3) IPR022803 - Ribosomal protein L5 domain; Mitochondrial ribosomal protein L5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTGTTGGGCGGCATGTCGCTGCCGGTACTGAGCGGCGCTGCGGCAGA |
| Internal bar code: | CGAACTGGATCATAAGTACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1762 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCCCATGCCAGTCATAGA |
| Suggested primer 2: | CGCATCGGTTAGCAAAGTCG |