| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.011865 |
| Chromosome: | chromosome 12 |
| Location: | 7249277 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558800 | (1 of 2) IPR000104//IPR017923 - Antifreeze protein, type I // Transcription factor IIS, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGGCCGCACTTCCACGGACACGGACACATGCACCACAGCGCCGCCG |
| Internal bar code: | CAATCTCTTTATTCGTTCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1675 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACCCGTTGTGTCGCTTG |
| Suggested primer 2: | GTGTGTGCGTGTGTGTGTTT |