| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.011891 |
| Chromosome: | chromosome 12 |
| Location: | 5128038 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526500 | (1 of 7) PTHR12743:SF0 - CYTOCHROME C-TYPE HEME LYASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCTGCGCCACTACGCGCTGCAAAGTAAGCCCTACCATGCCGCGTCCC |
| Internal bar code: | TCTCAAACGACCGTGCTCGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 10.3448 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCTTGATTGGGGTAGC |
| Suggested primer 2: | TCACACAGCTCACACTCGAC |