Insertion junction: CLIP2.011900_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.011900
Chromosome:chromosome 12
Location:5717131
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g532250 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGTGCTGCTGCAGGTGCGCCGCCGCCGCCGCCGCTAGGGTGCGGAGCG
Internal bar code:ATTGTTACGATACCAACGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3047
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAACATGTCGCTTCCTGG
Suggested primer 2:ATGCAAGCTAGCCCTTTCGT