Insertion junction: CLIP2.011913_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.011913
Chromosome:chromosome 10
Location:3108164
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g441550 MAM3B (1 of 5) K16302 - metal transporter CNNM (CNNM); Protein putatively involved in mitochondrial biogenesis CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCACACGACCGCTTCCGCCACCGCCACCGCCACCGGCGCCACCGCCA
Internal bar code:AGCGGGAAGCAAGCAGGTCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:954
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGTCGACTCAGGGTGATG
Suggested primer 2:CGCACAGACACACACACAAG