| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.011921 |
| Chromosome: | chromosome 6 |
| Location: | 5491754 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286350 | UMM6 | UbiE/COQ5 methyltransferase family protein; (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCGACCTGTGTGCGTGCCAGACACACACACACATATACATATGGCTG |
| Internal bar code: | ATGCCTGTTCAATATACAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGCTTTCGTTTCGGTTTT |
| Suggested primer 2: | CACCTCGAGCAGTTTCAGGT |