Insertion junction: CLIP2.011957_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.011957
Chromosome:chromosome 1
Location:3489045
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATCGTATATTATTTCACCACTTTCTTTATTTACGTAGTTCAGCCCTGCT
Internal bar code:CCTGCTGTAGGGGCACAAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:474
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGAATCACGCTGCGAATGAG
Suggested primer 2:TAACGCGTGACTTGACGACA