| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.011981 |
| Chromosome: | chromosome 4 |
| Location: | 2266184 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACCTATACGTTTCAGAAAGCGGCAGTTGACGAAAACTTGGGCAACA |
| Internal bar code: | AGTGGACCATCGTAATCGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 57.5 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCGAGATACAGACTCCAC |
| Suggested primer 2: | TCAGTCGTCTAGGTCCAGCA |