Insertion junction: CLIP2.012001_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.012001
Chromosome:chromosome 2
Location:6948181
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389134 (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) 3'UTR
Cre09.g389171 gene_edge/mRNA_edge/CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGACAGAGAGTGTCCTTCGAGTGATTTGACAAGACTATTTCGGATGAG
Internal bar code:TGGAGGCTCATGAATAGAGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:889
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTGGACACACCTTGGTTT
Suggested primer 2:GAGAGTCGTGTTTCTGGCCA