| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012005 |
| Chromosome: | chromosome 12 |
| Location: | 5389906 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529150 | PPP40,PP2C1,FAP314 | Flagellar Associated Protein 314; (1 of 1) PTHR13832:SF352 - PROTEIN PHOSPHATASE 2C 59-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCACGGACACTCAGTACTTGCGGCAGGTGCGGCAACACCCCTGGGGTTG |
| Internal bar code: | CAGTTAGTCAGGATTGGCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2084 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTACCATACTGCCCGAC |
| Suggested primer 2: | ATCGTGGGCCTGTTTGGAAT |