Insertion junction: CLIP2.012027_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.012027
Chromosome:chromosome 7
Location:5835533
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g353600 MMP7 Metalloproteinase of VMP family; (1 of 40) 3.4.24.38 - Gametolysin / Lysin intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCAAGAGGTTTGTGAACAGACTGTGAATGCCACCTGGCAAGCTCTGGG
Internal bar code:GACTGCGGCAGGGGTTATACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1843
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CATGGACAACGACCAGGTCA
Suggested primer 2:AACACAGACCAACACTGCCA