| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012032 |
| Chromosome: | chromosome 2 |
| Location: | 2738527 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093750 | NRX2 | Nucleoredoxin 2; (1 of 3) K17609 - nucleoredoxin [EC:1.8.1.8] (NXN) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTATTTGAGGTCGAAGTAAGGGTTGTCAAGCTGTGTCCGAATGACGGC |
| Internal bar code: | CCGTCGATGATCGGATTTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4618 |
| LEAP-Seq percent confirming: | 94.3182 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTAGAGCGATCGTCCC |
| Suggested primer 2: | CAGGATGACCAGGGTAGGGA |