| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.012039 |
| Chromosome: | chromosome 12 |
| Location: | 9075094 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544450 | CSE20 | Predicted protein of CSE family | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCACCTGGCTGGCCAGCACCTTCCAGGCGTTGGCGAAGGCGAGCTTG |
| Internal bar code: | GGTGTTTAATAACAACACACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGATCCCGTCACATACCG |
| Suggested primer 2: | GTACCGATACAGCCCAGTCG |