Insertion junction: CLIP2.012057_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.012057
Chromosome:chromosome 6
Location:3141122
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g275600 5'UTR
Cre06.g275650 RPN3 (1 of 1) K03033 - 26S proteasome regulatory subunit N3 (PSMD3, RPN3); 26S proteasome regulatory subunit 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACATGCAGTCTCCTCCCCCAAATCCAGTATATGTAGGACTTGGCAG
Internal bar code:AGCTCTCTTAAATTTATTAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:865
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACGAGGACGACTTCTGA
Suggested primer 2:CCGACAACTTCTTCCTGCCT