Insertion junction: CLIP2.012061_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.012061
Chromosome:chromosome 3
Location:6434217
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g193050 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCATGCTCAGGACTCAGGGGAGGACCTCACTCAAGGACGAACAAGCGCT
Internal bar code:TTCTTTATTTTTATATCTGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:4459
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TTGAGGAGGAGGACGAGGAG
Suggested primer 2:ACACGTACATGAAGCGCTGA