Insertion junction: CLIP2.012091_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.012091
Chromosome:chromosome 5
Location:3086333
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre24.g755597 Chlorophyll a /Pheophorbide a oxygenase-related protein; (1 of 2) 1.14.13.122 - Chlorophyllide-a oxygenase / Cholorophyll-b synthase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTACCGACGACGCGCCCTCGCCTTTTATCGAGCCCTTGATTCGCGGCC
Internal bar code:ATGGGTGAGGGAGCGGTGAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:831
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCCAGAGTCTCAGGTGGTTC
Suggested primer 2:GTTCGCAGTGCAAAGAAGCA