Insertion junction: CLIP2.012091_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.012091
Chromosome:chromosome 16
Location:5676450
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCCCCCCCACGCTGACTTGCACGTCTGCCACTCCGTCCCTCATATGC
Internal bar code:AAATGCTCCATATAGCGAATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:668
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGACACACTCCATGCCCATG
Suggested primer 2:TGTAGTGCTGCAGGTCTGTG