| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012095 |
| Chromosome: | chromosome 1 |
| Location: | 5030001 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034900 | CDPK5 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1; Calcium/calmodulin-dependent protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAGATATCATGCTGGCGGTGGGTGCGGGCGGGGGTCGGGGCTTGGGGC |
| Internal bar code: | CCGGTCTACACTCCTAATTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2139 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCGCCACATCACTTGAC |
| Suggested primer 2: | GATGAATCCAACACGGCAGC |