Insertion junction: CLIP2.012106_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.012106
Chromosome:chromosome 8
Location:1854949
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g367250 FAP176 (1 of 239) IPR016024 - Armadillo-type fold; Flagellar Associated Protein 176 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCTCCGCCTCCACCCCCATCGCCTCCCTGCCTGTACTCCGCTAGT
Internal bar code:TTTTTTTTCGGGATGCGGAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1693
LEAP-Seq percent confirming:64.2857
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGGCAACCATTTCGCAAC
Suggested primer 2:GAAATGGTAGACGGTGGGCA