Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.012109 |
Chromosome: | chromosome 17 |
Location: | 3954362 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g728200 | (1 of 1) K11664 - vacuolar protein sorting-associated protein 72 (VPS72, TCFL1, YL1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTTTCGTCTGAGTCTCATTAGCGTTACCCACAAAGCGTGTCGGGGTTC |
Internal bar code: | TGGGGTACCCATAGGTCGGGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2831 |
LEAP-Seq percent confirming: | 93.9394 |
LEAP-Seq n confirming: | 31 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCCTCATTCATCGCGAAC |
Suggested primer 2: | GCTGGAAGGACACGACATGA |