Insertion junction: CLIP2.012109_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.012109
Chromosome:chromosome 17
Location:3954362
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g728200 (1 of 1) K11664 - vacuolar protein sorting-associated protein 72 (VPS72, TCFL1, YL1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTTCGTCTGAGTCTCATTAGCGTTACCCACAAAGCGTGTCGGGGTTC
Internal bar code:TGGGGTACCCATAGGTCGGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2831
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCCTCATTCATCGCGAAC
Suggested primer 2:GCTGGAAGGACACGACATGA