| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.012110 |
| Chromosome: | chromosome 16 |
| Location: | 2039882 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656950 | (1 of 1) PTHR13140:SF300 - PROTEIN TAG-278-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTGTTGACTCGCCTGGACGCGGTGGAGGCTAGCCGCAAGCAGCTTGA |
| Internal bar code: | GGTCTCGATAAGAGACTTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4134 |
| LEAP-Seq percent confirming: | 68.75 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCTTCCGAGACGTTCAA |
| Suggested primer 2: | CTGCAAGCCACCAAGGAGTA |