Insertion junction: CLIP2.012112_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.012112
Chromosome:chromosome 8
Location:1107697
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g362650 IFT140 Intraflagellar Transport Protein 140; (1 of 1) PTHR15722//PTHR15722:SF7 - IFT140/172-RELATED // INTRAFLAGELLAR TRANSPORT PROTEIN 140 HOMOLOG intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGAGCAATAGGAGCAGCAGGAGCCGGGCATGCGGCGTCAGAGGTCCAGA
Internal bar code:GCAGGCGTTATAAGCAGGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:2753
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCCATTTGAACAGCTTGG
Suggested primer 2:CACCTTGGCGAACAGGTAGT