| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012130 |
| Chromosome: | chromosome 12 |
| Location: | 4016101 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516000 | (1 of 2) PF07978 - NIPSNAP (NIPSNAP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCAGGAAGCCCACCATCTGCGCCATGGCCCCTGCCGCGAGCTAACTT |
| Internal bar code: | GAGCCATTTCGGGGCGCGTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5398 |
| LEAP-Seq percent confirming: | 98.5916 |
| LEAP-Seq n confirming: | 140 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 142 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAATCACTCTCACCAGGT |
| Suggested primer 2: | GGGAAGCGTAGGTATCAGCC |