| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012134 |
| Chromosome: | chromosome 5 |
| Location: | 1557880 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234666 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTGGGCAGCGGCGGCGGTGCAGGGGAGGAGCGCCCGCAGGCAGAGCC |
| Internal bar code: | GTGTACGCGATCGGTCGAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4770 |
| LEAP-Seq percent confirming: | 99.3506 |
| LEAP-Seq n confirming: | 153 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 154 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTGCAGCAAACGAGAAA |
| Suggested primer 2: | CACCGTTGCTACGTTTGCAA |