Insertion junction: CLIP2.012146_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.012146
Chromosome:chromosome 16
Location:2544104
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g660800 TIM44 (1 of 1) K17804 - mitochondrial import inner membrane translocase subunit TIM44 (TIM44); Mitochondrial inner membrane translocase subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACCATTGCAGGAGCAGGCTAACATACATGCAAGCAAATACGTGTAGC
Internal bar code:CTCTACCTTTAGCCCTAATAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:946
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CGACCTGGATACGCTGTCTC
Suggested primer 2:TGGACTGCCACATCATGTCC