| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.012151 |
| Chromosome: | chromosome 1 |
| Location: | 6700695 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g047850 | (1 of 1) 4.2.1.145 - Capreomycidine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTAGTGGGCTGGAGCACCAGTGGAAAATGCACGGCGTGGCAGAGGAG |
| Internal bar code: | GGATGTGGTGGGGGACTTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2683 |
| LEAP-Seq percent confirming: | 95.8904 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGGCGGGGGATAAAAAC |
| Suggested primer 2: | GCGGTACATCTCATCGCTGA |