Insertion junction: CLIP2.012157_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.012157
Chromosome:chromosome 6
Location:3780487
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278170 FAP237,FAS7,FAS16,FLA11 Flagellar Associated Protein 237; (1 of 1) PTHR10900:SF77 - PROTEIN F26E4.7, ISOFORM A CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGGGACCGTCGAGACCGGCACCGTGCCGGTACCGGAAGACGACGGCG
Internal bar code:GATGTTCTGCGGTAACTACTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1271
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:ACTCCGCCAAGGTCATCAAG
Suggested primer 2:GTTGGCATTGTACGAGTCGC