Insertion junction: CLIP2.012160_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.012160
Chromosome:chromosome 2
Location:5989379
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g118450 DNJ28 (1 of 1) IPR000104//IPR001623//IPR012336 - Antifreeze protein, type I // DnaJ domain // Thioredoxin-like fold; DnaJ-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACACCTGCCCGTCACATACTCCAGCGCTCCCGGCTCGTCGTCCGTCT
Internal bar code:TACTCTTACAATACTCGGGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1826
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACCTGGTTCGACTCCAC
Suggested primer 2:CGAGATACCCCACTGCATCC