Insertion junction: CLIP2.012188_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.012188
Chromosome:chromosome 7
Location:2771718
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g800794 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCTGGGAGATGCAACAACGGGGCTTCTGAAGTTGTGCGAGGCAGGGA
Internal bar code:ACTAATCTAGTTGTACACTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:558
LEAP-Seq percent confirming:20.8333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAATCCTGCGTGATGAAC
Suggested primer 2:CTCCGACTCCCTATTTCGCC