Insertion junction: CLIP2.012214_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.012214
Chromosome:chromosome 9
Location:6020937
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412500 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGAATGCACCACATACAGCCCACGCGCGCTGATACAGCAATTCGTACTT
Internal bar code:CAGCTAACCGCAGCTCGGTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3526
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:130
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:130

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATTTTGTTCGCACAGGG
Suggested primer 2:AACTGCGTCTGGTGGTACTG