Insertion junction: CLIP2.012220_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.012220
Chromosome:chromosome 10
Location:608597
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421700 GPDH1,GPD4 Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase; (1 of 4) 1.1.1.8 - Glycerol-3-phosphate dehydrogenase (NAD(+)) / NADH-dihydroxyacetone phosphate reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACACACACGCGCAACTCGTGCACACGCACGCACACGATCGCTTCGTACA
Internal bar code:TTCTTTAAAAAGCAGTTGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1325
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGGTATGTACGCCCCTG
Suggested primer 2:CACACACACGTTCTGGCTTG