| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012223 |
| Chromosome: | chromosome 12 |
| Location: | 5804262 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532950 | (1 of 4) PF00060//PF00497 - Ligand-gated ion channel (Lig_chan) // Bacterial extracellular solute-binding proteins, family 3 (SBP_bac_3); Ligand-gated ion channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCCAGTTCCAAACCTGGATCATAGGGTTGAGGGAAGCGGGGCACGCA |
| Internal bar code: | TGTGGCGCGTTCTGGGACTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1835 |
| LEAP-Seq percent confirming: | 98.913 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTGGACTGCAACCTTCG |
| Suggested primer 2: | ACCATCCACTCCCATCCGTA |