| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.012235 |
| Chromosome: | chromosome 2 |
| Location: | 1680206 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085750 | (1 of 8) PF13416 - Bacterial extracellular solute-binding protein (SBP_bac_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCCCCCTCCACCTCCCGAGTTGAGTTACCCAGCCCCCCGCCCCCAGC |
| Internal bar code: | GACCTGATCGCTCCTCAAAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2612 |
| LEAP-Seq percent confirming: | 79.4118 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGAAGAAGGCGGAGTCG |
| Suggested primer 2: | ATGCATATCGTACCGGCGTT |