| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.012253 |
| Chromosome: | chromosome 6 |
| Location: | 35412 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g248800 | (1 of 1) PTHR31109:SF2 - PROTEIN FAM207A | CDS | |
| Cre06.g248850 | (1 of 1) K08827 - serine/threonine-protein kinase PRP4 [EC:2.7.11.1] (PRPF4B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAAGGCTGCAAATTCCAGCAAAATGCAGGAATGACAACCAAAATGCA |
| Internal bar code: | CTTTATGATGGGTCGAAACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6526 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGGCCCTTCACCAAAAA |
| Suggested primer 2: | TGACAGTCGACCAAGACACG |