Insertion junction: CLIP2.012286_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.012286
Chromosome:chromosome 2
Location:4084406
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g103350 (1 of 1) 3.5.1.99 - Fatty acid amide hydrolase / Oleamide hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACAGACACTCTCTCGCTCTCTGCGCCACACACCCCAGCAACCCTGCCC
Internal bar code:GGCTGGAGCGGATTTAGGCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:416
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCACCCTCCACACACTC
Suggested primer 2:CCGCCTGCACAACATTTCAA