| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.012312 |
| Chromosome: | chromosome 16 |
| Location: | 7542539 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683147 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAACCCAAGGGCCGCAGGCTTCTGGCGCCCTCTCAGCCCGCTATTGC |
| Internal bar code: | CAGCTCGAGCGGCCGTCTATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1730 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCGTGACATCCCCAAAGC |
| Suggested primer 2: | AATGGCTTGCTCCACATCCA |